UNIT 13.1 Overview of Protein Phosphorylation

  1. Bartholomew M. Sefton1,
  2. Shirish Shenolikar2

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps1301s00

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Sefton, B. M. and Shenolikar, S. 2001. Overview of Protein Phosphorylation. Current Protocols in Protein Science. 00:13.1:13.1.1–13.1.5.

Author Information

  1. 1

    The Salk Institute, San Diego, California

  2. 2

    Duke University Medical Center, Durham, North Carolina

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1995


This overview provides a history of protein phosphorylation research and provides the reader with an understanding of how and why labeling studies are performed. The various sites of protein phosphorylation are described along with the roles of the many kinases and phosphatases that regulate phosphorylation. Methods for detecting unlabeled phosphoamino acids, including high-voltage electrophoresis on thin-layer cellulose acetate plates, gel-shift assays, and the use of anti-phosphopeptide antibodies.