UNIT 13.1 Overview of Protein Phosphorylation
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Sefton, B. M. and Shenolikar, S. 2001. Overview of Protein Phosphorylation. Current Protocols in Protein Science. 00:13.1:13.1.1–13.1.5.
- Published Online: 1 MAY 2001
- Published Print: JUN 1995
This overview provides a history of protein phosphorylation research and provides the reader with an understanding of how and why labeling studies are performed. The various sites of protein phosphorylation are described along with the roles of the many kinases and phosphatases that regulate phosphorylation. Methods for detecting unlabeled phosphoamino acids, including high-voltage electrophoresis on thin-layer cellulose acetate plates, gel-shift assays, and the use of anti-phosphopeptide antibodies.