Unit

UNIT 13.2 Labeling Cultured Cells with 32Pi and Preparing Cell Lysates for Immunoprecipitation

  1. Bartholomew M. Sefton

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps1302s10

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Sefton, B. M. 2001. Labeling Cultured Cells with 32Pi and Preparing Cell Lysates for Immunoprecipitation. Current Protocols in Protein Science. 10:13.2:13.2.1–13.2.8.

Author Information

  1. The Salk Institute, San Diego, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1997

Abstract

This unit describes 32PI labeling and lysis of cultured cells to be used for subsequent immunoprecipitation of proteins. The procedure is suitable for insect, avian, and mammalian cells and can be used with both adherent and nonadherent cultures. The protocol described is 32PI labeling of adherent or nonadherent (e.g., hematopoietic) cells with subsequent lysis in a detergent buffer. More rigorous lysis conditions to be used for working with proteins that are difficult to solubilize are also described.