Unit

UNIT 13.11 Tyrosine Phosphorylation Enrichment and Subsequent Analysis by MALDI-TOF/TOF MS/MS and LC-ESI-IT-MS/MS

  1. Mark R. Condina1,
  2. Manuela Klingler-Hoffmann2,
  3. Peter Hoffmann1

Published Online: 1 NOV 2010

DOI: 10.1002/0471140864.ps1311s62

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Condina, M. R., Klingler-Hoffmann, M. and Hoffmann, P. 2010. Tyrosine Phosphorylation Enrichment and Subsequent Analysis by MALDI-TOF/TOF MS/MS and LC-ESI-IT-MS/MS. Current Protocols in Protein Science. 62:13.11:13.11.1–13.11.26.

Author Information

  1. 1

    Adelaide Proteomics Centre, School of Molecular and Biomedical Science, University of Adelaide, Adelaide, Australia

  2. 2

    Chemokine Biology Laboratory, School of Molecular and Biomedical Science, University of Adelaide, Adelaide, Australia

Publication History

  1. Published Online: 1 NOV 2010
  2. Published Print: NOV 2010

Abstract

This unit describes methods to detect, identify, and characterize tyrosine phosphorylation in proteins by mass spectrometry, including sample preparation methods, enrichment strategies using phosphotyrosine-specific antibodies, and chromatographic separation methods. Curr. Protoc. Protein Sci. 62:13.11.1-13.11.26. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • ESI-IT-MS/MS;
  • liquid chromatography;
  • MALDI;
  • matrix preparation;
  • tyrosine phosphorylation