UNIT 13.11 Tyrosine Phosphorylation Enrichment and Subsequent Analysis by MALDI-TOF/TOF MS/MS and LC-ESI-IT-MS/MS
Published Online: 1 NOV 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Condina, M. R., Klingler-Hoffmann, M. and Hoffmann, P. 2010. Tyrosine Phosphorylation Enrichment and Subsequent Analysis by MALDI-TOF/TOF MS/MS and LC-ESI-IT-MS/MS. Current Protocols in Protein Science. 62:13.11:13.11.1–13.11.26.
- Published Online: 1 NOV 2010
- Published Print: NOV 2010
This unit describes methods to detect, identify, and characterize tyrosine phosphorylation in proteins by mass spectrometry, including sample preparation methods, enrichment strategies using phosphotyrosine-specific antibodies, and chromatographic separation methods. Curr. Protoc. Protein Sci. 62:13.11.1-13.11.26. © 2010 by John Wiley & Sons, Inc.
- liquid chromatography;
- matrix preparation;
- tyrosine phosphorylation