Unit

UNIT 14.4 Analysis of Oxidative Modification of Proteins

  1. Liang-Jun Yan

Published Online: 1 APR 2009

DOI: 10.1002/0471140864.ps1404s56

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Yan, L.-J. 2009. Analysis of Oxidative Modification of Proteins. Current Protocols in Protein Science. 56:14.4:14.4.1–14.4.28.

Author Information

  1. Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, Fort Worth, Texas

Publication History

  1. Published Online: 1 APR 2009
  2. Published Print: APR 2009

Abstract

Proteins are targets of oxidative modification. This unit describes detailed procedures for the analysis of popular indices of protein oxidation including protein carbonyl formation, loss of protein thiols, and nitrotyrosine and dityrosine formation, as well as isoaspartate formation. Procedures are detailed for the analysis of protein carbonyls labeled with 2,4-dinitrophenylhydrazine, tritiated sodium borohydride, and biotin-hydrazide, followed by detection measurements that are based on the distinguishing feature of each labeling chemical. Methods are outlined for the determination of protein cysteine oxidation by quantifying the loss of free protein thiols using radiolabeled [14C]-iodoacetamide. Protocols are described for the measurement of protein dityrosine by gas chromatography/mass spectrometry, as are the details for the detection of protein nitrotyrosine by a competitive ELISA approach. Finally, methods are described for the quantification of protein-bound isoaspartate using protein-l-isoaspartyl methyltransferase that converts aberrant l-isoaspartyl residues in peptides and proteins to normal aspartyl residues. Curr. Protoc. Protein Sci. 56:14.4.1-14.4.28. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • protein oxidative modification;
  • carbonylation;
  • cysteine;
  • dityrosine;
  • isoaspartate;
  • nitrotyrosine