UNIT 14.6 Analysis of Protein S-Nitrosylation
Published Online: 1 FEB 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Schonhoff, C. M. and Benhar, M. 2011. Analysis of Protein S-Nitrosylation. Current Protocols in Protein Science. 63:14.6:14.6.1–14.6.21.
- Published Online: 1 FEB 2011
- Published Print: FEB 2011
S-Nitrosylation, the redox-based modification of cysteine thiol side chains by nitric oxide, is a dynamic and reversible post-translational modification of proteins that subserves many important cellular functions. Analysis of protein S-nitrosylation is often challenging due to methodological limitations and the effects of various chemical and physical parameters. Despite these technical challenges, a growing number of useful methods are now available to analyze protein S-nitrosylation. In this unit, several important methods to measure protein S-nitrosylation and denitrosylation are discussed and evaluated. Recommendations are given regarding the potential and the applicability of the methods discussed. Curr. Protoc. Protein Sci. 63:14.6.1-14.6.21. © 2011 by John Wiley & Sons, Inc.
- nitric oxide;
- Saville-Griess assay;
- chemical reduction/chemiluminescence;
- DAN assay;
- biotin-switch method