UNIT 16.1 Overview of Peptide and Protein Analysis by Mass Spectrometry
Published Online: 1 NOV 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Zhang, G., Annan, R. S., Carr, S. A. and Neubert, T. A. 2010. Overview of Peptide and Protein Analysis by Mass Spectrometry. Current Protocols in Protein Science. 62:16.1:16.1.1–16.1.30.
- Published Online: 1 NOV 2010
- Published Print: NOV 2010
Mass spectrometry is an indispensable tool for peptide and protein analysis owing to its speed, sensitivity, and versatility. It can be used to determine amino acid sequences of peptides, and to characterize a wide variety of post-translational modifications such as phosphorylation and glycosylation. Mass spectrometry can also be used to determine absolute and relative protein quantities, and can identify and quantify thousands of proteins from complex samples, which makes it an extremely powerful tool for systems biology studies. The main goals of this unit are to familiarize peptide and protein chemists and biologists with the types of mass spectrometers that are appropriate for the majority of their analytical needs, to describe the kinds of experiments that can be performed with these instruments on a routine basis, and to discuss the kinds of information that these experiments provide. Curr. Protoc. Protein Sci. 62:16.1.1-16.1.30. © 2010 by John Wiley & Sons, Inc.
- mass spectrometry;