Unit

UNIT 16.2 Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Analysis of Peptides

  1. William J. Henzel,
  2. John T. Stults

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps1602s04

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Henzel, W. J. and Stults, J. T. 2001. Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Analysis of Peptides. Current Protocols in Protein Science. 4:16.2:16.2.1–16.2.11.

Author Information

  1. Genentech, Inc., South San Francisco, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1996

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Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is one of the most useful techniques for determining the mass of biomolecules, with exceptional capabilities for mass analysis of peptides. Relative to other ionization techniques, it provides high sensitivity and excellent tolerance of salt and other common buffer components. Routine detection limits for peptides are in the subpicomole range. The ions commonly observed are the protonated molecules (M+H+), which makes data analysis relatively easy. This overview discusses instrument configuration and calibration, sample preparation, along with specific approaches for analyzing peptide mixtures, synthetic peptides, and chemical modifications of peptides.