Unit

UNIT 16.5 Searching Sequence Databases Over the Internet: Protein Identification Using MS-Fit

  1. C.R. Jiménez,
  2. L. Huang,
  3. Y. Qiu,
  4. A.L. Burlingame

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps1605s14

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Jiménez, C., Huang, L., Qiu, Y. and Burlingame, A. 2001. Searching Sequence Databases Over the Internet: Protein Identification Using MS-Fit. Current Protocols in Protein Science. 14:16.5:16.5.1–16.5.6.

Author Information

  1. University of California San Francisco, San Francisco, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1998

Abstract

Peptide fingerprint mass mapping is widely used for initial identification of proteins separated by gel electrophoresis. This method involves the enzymatic in-gel digestion of proteins to generate peptides, followed by mass measurement of the cleaved peptides. The experimental mass values are then compared with theoretical values from protein databases, calculated using the cleavage specificity of the enzyme. This unit describes the use of the search program MS-Fit to identify the closest match or matches.