Unit

UNIT 16.8 Introducing Samples Directly into Electrospray Ionization Mass Spectrometers Using a Nanospray Interface

  1. Terry D. Lee,
  2. Roger E. Moore,
  3. Mary K. Young

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps1608s22

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Lee, T. D., Moore, R. E. and Young, M. K. 2001. Introducing Samples Directly into Electrospray Ionization Mass Spectrometers Using a Nanospray Interface. Current Protocols in Protein Science. 22:16.8:16.8.1–16.8.5.

Author Information

  1. Beckman Research Institute of the City of Hope, Duarte, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 2000

This is not the most recent version of the article. View current version (5 NOV 2013)

Abstract

Electrospray (ES) is a concentration-dependent phenomenon, and operation at high flow rates results in a corresponding high rate of sample consumption. Sensitivity increases are most readily achieved by decreasing the flow rate of sample solution into the electrospray source. In recent years, a number of methodologies have been developed to achieve ES analyses at sub-ml/min flow rates. There are a large number of microscale ES devices currently in use, but this unit describes in detail a micro-scale interface for direct liquid introduction without separation.