UNIT 16.8 Introducing Samples Directly into Electrospray Ionization Mass Spectrometers Using a Nanospray Interface
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Lee, T. D., Moore, R. E. and Young, M. K. 2001. Introducing Samples Directly into Electrospray Ionization Mass Spectrometers Using a Nanospray Interface. Current Protocols in Protein Science. 22:16.8:16.8.1–16.8.5.
- Published Online: 1 MAY 2001
- Published Print: DEC 2000
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Electrospray (ES) is a concentration-dependent phenomenon, and operation at high flow rates results in a corresponding high rate of sample consumption. Sensitivity increases are most readily achieved by decreasing the flow rate of sample solution into the electrospray source. In recent years, a number of methodologies have been developed to achieve ES analyses at sub-ml/min flow rates. There are a large number of microscale ES devices currently in use, but this unit describes in detail a micro-scale interface for direct liquid introduction without separation.