Unit

UNIT 16.8 Introducing Samples Directly into Electrospray Ionization Mass Spectrometers by Direct Infusion Using a Nanoelectrospray Interface

  1. Terry D. Lee,
  2. Roger E. Moore,
  3. Denise Keen

Published Online: 5 NOV 2013

DOI: 10.1002/0471140864.ps1608s74

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Lee, T. D., Moore, R. E. and Keen, D. 2013. Introducing Samples Directly into Electrospray Ionization Mass Spectrometers by Direct Infusion Using a Nanoelectrospray Interface. Current Protocols in Protein Science. 74:16.8:16.8.1–16.8.7.

Author Information

  1. Beckman Research Institute of the City of Hope, Duarte, California

Publication History

  1. Published Online: 5 NOV 2013

Abstract

Procedures are described for constructing and using a microscale electrospray interface for direct infusion of samples into mass spectrometers. The sensitivity of the nanospray interface is a result of greatly reducing the flow of sample solution while preserving the analyte signal intensity. The described methodology provides a simple and robust way to analyze individual purified peptide and protein samples, i.e., samples that do not require liquid chromatography separation. Curr. Protoc. Protein Sci. 74:16.8.1-16.8.7. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • mass spectrometry;
  • nanoelectrospray;
  • microscale electrospray interface