UNIT 16.8 Introducing Samples Directly into Electrospray Ionization Mass Spectrometers by Direct Infusion Using a Nanoelectrospray Interface
Published Online: 5 NOV 2013
Copyright © 2013 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Lee, T. D., Moore, R. E. and Keen, D. 2013. Introducing Samples Directly into Electrospray Ionization Mass Spectrometers by Direct Infusion Using a Nanoelectrospray Interface. Current Protocols in Protein Science. 74:16.8:16.8.1–16.8.7.
- Published Online: 5 NOV 2013
Procedures are described for constructing and using a microscale electrospray interface for direct infusion of samples into mass spectrometers. The sensitivity of the nanospray interface is a result of greatly reducing the flow of sample solution while preserving the analyte signal intensity. The described methodology provides a simple and robust way to analyze individual purified peptide and protein samples, i.e., samples that do not require liquid chromatography separation. Curr. Protoc. Protein Sci. 74:16.8.1-16.8.7. © 2013 by John Wiley & Sons, Inc.
- mass spectrometry;
- microscale electrospray interface