Unit

UNIT 16.10 Protein Identification Using a Quadrupole Ion Trap Mass Spectrometer and SEQUEST Database Matching

  1. Roger E. Moore,
  2. Mary K. Young,
  3. Terry D. Lee

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps1610s22

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Moore, R. E., Young, M. K. and Lee, T. D. 2001. Protein Identification Using a Quadrupole Ion Trap Mass Spectrometer and SEQUEST Database Matching. Current Protocols in Protein Science. 22:16.10:16.10.1–16.10.9.

Author Information

  1. Beckman Research Institute of the City of Hope, Duarte, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 2000

Abstract

A procedure is described for rapid, sensitive protein identification utilizing liquid chromatography-- tandem mass spectrometry. The analysis is performed on mixtures of peptides obtained by enzyme digestion. The SEQUEST computer program is used to match the sequence information in the spectra to a database of known protein sequences.