UNIT 17.3 Principles of Macromolecular X-Ray Crystallography

  1. Alison B. Hickman,
  2. David R. Davies

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps1703s10

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Hickman, A. B. and Davies, D. R. 2001. Principles of Macromolecular X-Ray Crystallography. Current Protocols in Protein Science. 10:17.3:17.3.1–17.3.15.

Author Information

  1. National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1997


X-ray crystallography is one of the major tools available for protein structural analysis. This unit provides an introductory review of the principles of X-ray crystallography that covers how the image is generated and analyzed. The second half of the unit describes strategies for producing crystals of protein, including methods for dealing with proteins that do not easily form crystals.