Unit
UNIT 17.5 Introduction to NMR of Proteins
Published Online: 1 MAY 2001
DOI: 10.1002/0471140864.ps1705s19
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
Additional Information
How to Cite
Edwards, A. J. and Reid, D. 2001. Introduction to NMR of Proteins. Current Protocols in Protein Science. 19:17.5:17.5.1–17.5.39.
Publication History
- Published Online: 1 MAY 2001
- Published Print: MAR 2000
This is not the most recent version of the article. View current version (1 APR 2011)
Abstract
This unit aims to overview the application of NMR spectroscopy to proteins. It is not intended to provide an exhaustive “how to” guide, but rather to give a flavor of both well-established and emerging NMR techniques used in the elucidation of protein structure. It starts with a brief introduction to the basic principles of NMR and the information provided by this technique, and goes on to discuss the instrumentation involved, spectral assignment methods for small and large proteins, and the utility of other spin active nuclei (e.g., 13C and 15N) to aid assignment of the latter.
