Unit

UNIT 17.6 Investigating Solution-Phase Protein Structure and Dynamics by Hydrogen Exchange Mass Spectrometry

  1. Christopher R. Morgan,
  2. John R. Engen

Published Online: 1 NOV 2009

DOI: 10.1002/0471140864.ps1706s58

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Morgan, C. R. and Engen, J. R. 2009. Investigating Solution-Phase Protein Structure and Dynamics by Hydrogen Exchange Mass Spectrometry. Current Protocols in Protein Science. 58:17.6:17.6.1–17.6.17.

Author Information

  1. Northeastern University, Boston, Massachusetts

Publication History

  1. Published Online: 1 NOV 2009
  2. Published Print: NOV 2009

Abstract

By taking advantage of labeling methods such as hydrogen exchange (HX), many details about protein conformation, dynamics, and interactions can be revealed by mass spectrometry. In this unit, hydrogen exchange theory is discussed as it applies to HX-MS protocols, the practice of HX-MS including data analysis and interpretation is explained in detail, and recent advancements in technology which greatly increase the depth of information gained from the technique are highlighted. Curr. Protoc. Protein Sci. 58:17.6.1-17.9.17. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • proteins;
  • mass spectrometry;
  • hydrogen exchange