UNIT 17.10 Application of Dynamic Light Scattering in Protein Crystallization
Published Online: 1 AUG 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Proteau, A., Shi, R. and Cygler, M. 2010. Application of Dynamic Light Scattering in Protein Crystallization. Current Protocols in Protein Science. 61:17.10:17.10.1–17.10.9.
- Published Online: 1 AUG 2010
- Published Print: AUG 2010
Success in determining the three-dimensional structure of a macromolecule by X-ray diffraction methods depends critically on the ability to obtain well ordered crystals of the macromolecule in question. Predisposition to crystallization correlates with the homogeneity of the molecules in solution. Dynamic light scattering (DLS) is particularly well suited for evaluating protein homogeneity under multiple conditions and at concentrations commensurate with crystallization conditions. This unit presents a typical protocol for DLS measurements of a protein sample, and describes approaches to improve protein homogeneity in solution. Curr. Protoc. Protein Sci. 61:17.10.1-17.10.9. © 2010 by John Wiley & Sons, Inc.
- dynamic light scattering;
- crystallization of proteins;