Unit

UNIT 17.10 Application of Dynamic Light Scattering in Protein Crystallization

  1. Ariane Proteau1,
  2. Rong Shi1,
  3. Miroslaw Cygler1,2

Published Online: 1 AUG 2010

DOI: 10.1002/0471140864.ps1710s61

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Proteau, A., Shi, R. and Cygler, M. 2010. Application of Dynamic Light Scattering in Protein Crystallization. Current Protocols in Protein Science. 61:17.10:17.10.1–17.10.9.

Author Information

  1. 1

    Department of Biochemistry, McGill University, Montreal, Quebec, Canada

  2. 2

    Biotechnology Research Institute, Montreal, Quebec, Canada

Publication History

  1. Published Online: 1 AUG 2010
  2. Published Print: AUG 2010

Abstract

Success in determining the three-dimensional structure of a macromolecule by X-ray diffraction methods depends critically on the ability to obtain well ordered crystals of the macromolecule in question. Predisposition to crystallization correlates with the homogeneity of the molecules in solution. Dynamic light scattering (DLS) is particularly well suited for evaluating protein homogeneity under multiple conditions and at concentrations commensurate with crystallization conditions. This unit presents a typical protocol for DLS measurements of a protein sample, and describes approaches to improve protein homogeneity in solution. Curr. Protoc. Protein Sci. 61:17.10.1-17.10.9. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • dynamic light scattering;
  • crystallization of proteins;
  • crystallography