UNIT 18.2 Combinatorial Peptide Synthesis on a Microchip
Published Online: 1 AUG 2009
Copyright © 2009 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Schirwitz, C., Block, I., König, K., Nesterov, A., Fernandez, S., Felgenhauer, T., Leibe, K., Torralba, G., Hausmann, M., Lindenstruth, V., Stadler, V., Breitling, F. and Bischoff, F. R. 2009. Combinatorial Peptide Synthesis on a Microchip. Current Protocols in Protein Science. 57:18.2.1–18.2.13.
- Published Online: 1 AUG 2009
- Published Print: AUG 2009
Microchips are used in the combinatorial synthesis of peptide arrays by means of amino acid microparticle deposition. The surface of custom-built microchips can be equipped with an amino-modified poly(ethylene glycol)methacrylate (PEGMA) graft polymer coating, which permits high loading of functional groups and resists nonspecific protein adsorption. Specific microparticles that are addressed to the polymer-coated microchip surface in a well defined pattern release preactivated amino acids upon melting, and thus allow combinatorial synthesis of high-complexity peptide arrays directly on the chip surface. Currently, arrays with densities of up to 40,000 peptide spots/cm
Keywords: solid phase peptide synthesis; peptide array; combinatorial synthesis; atom transfer radical polymerization (ATRP); amino acid particles