UNIT 18.6 Disulfide Bond Formation in Peptides

  1. Lin Chen1,
  2. Ioana Annis2,
  3. George Barany3

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps1806s23

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Chen, L., Annis, I. and Barany, G. 2001. Disulfide Bond Formation in Peptides. Current Protocols in Protein Science. 23:18.6:18.6.1–18.6.19.

Author Information

  1. 1

    AxCell Biosciences Corporation, Newtown, Pennsylvania

  2. 2

    Union Carbide Corporation, Bound Brook, New Jersey

  3. 3

    University of Minnesota, Minneapolis, Minnesota

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 2001


The formation of disulfide bridges is often a crucial final stage in peptide synthesis. There is compelling evidence that the disulfide pattern can be critical in the folding and structural stabilization of many natural peptide and protein sequences, while the artificial introduction of disulfide bridges into natural or designed peptides may often improve biological activities/specificities and stabilities. This unit provides a highly selective, albeit state-of-the-art, menu of procedures that can be performed to establish intramolecular or intermolecular disulfide bridges in targets of varying complexities.