Unit

UNIT 18.9 Phage Display for Generating Peptide Reagents

  1. Jennifer R. Brigati1,
  2. Tatiana I. Samoylova2,
  3. Prashanth K. Jayanna3,
  4. Valery A. Petrenko3

Published Online: 1 FEB 2008

DOI: 10.1002/0471140864.ps1809s51

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Brigati, J. R., Samoylova, T. I., Jayanna, P. K. and Petrenko, V. A. 2008. Phage Display for Generating Peptide Reagents. Current Protocols in Protein Science. 51:18.9:18.9.1–18.9.27.

Author Information

  1. 1

    Division of Natural Sciences, Maryville College, Maryville, Tennessee

  2. 2

    Scott-Ritchey Research Center, College of Veterinary Medicine, Auburn University, Auburn, Alabama

  3. 3

    Department of Pathobiology, Auburn University, Alabama

Publication History

  1. Published Online: 1 FEB 2008
  2. Published Print: FEB 2008

Abstract

This unit presents detailed protocols for selection and propagation of landscape phages, which are fusions of filamentous phage fd (or its close relatives M13 and f1) and foreign DNA that result in chimeric phage virions with foreign peptides (8 to 9 amino acids long) covering the entire surface of the phage particles. These landscape phages bind specifically to mammalian and bacterial cells, spores, or discrete molecular targets. Curr. Protoc. Protein Sci. 51:18.9.1-18.9.27. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • phage display;
  • landscape phage;
  • affinity selection;
  • major coat protein;
  • diagnostic probe;
  • detection;
  • cell-specific probes