Unit

UNIT 18.10 Utilizing Peptide SPOT Arrays to Identify Protein Interactions

  1. Ian Cushman

Published Online: 1 FEB 2008

DOI: 10.1002/0471140864.ps1810s51

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Cushman, I. 2008. Utilizing Peptide SPOT Arrays to Identify Protein Interactions. Current Protocols in Protein Science. 51:18.10:18.10.1–18.10.9.

Author Information

  1. Department of Pharmacology, Duke University, Durham, North Carolina

Publication History

  1. Published Online: 1 FEB 2008
  2. Published Print: FEB 2008

Abstract

SPOT arrays consist of synthesized peptides 12- to 18-amino acids long, with overlapping sequences that cover the entire sequence of a protein, covalently linked to a solid support. This unit describes how to construct peptide SPOT arrays, biotinylate recombinant proteins, and conduct overlay assays to identify binding interactions. In addition, directions describing how to analyze results to determine single amino acid binding contributions are included. The two techniques in this unit describe how to scan protein sequences to find binding motifs and how to conduct site-directed mutagenesis studies. Curr. Protoc. Protein Sci. 51:18.10.1-18.10.9. © 2008 by John Wiley & Sons, Inc.

Keywords:

  • peptides;
  • arrays;
  • protein-protein interactions