UNIT 18.10 Utilizing Peptide SPOT Arrays to Identify Protein Interactions
Published Online: 1 FEB 2008
Copyright © 2008 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Cushman, I. 2008. Utilizing Peptide SPOT Arrays to Identify Protein Interactions. Current Protocols in Protein Science. 51:18.10:18.10.1–18.10.9.
- Published Online: 1 FEB 2008
- Published Print: FEB 2008
SPOT arrays consist of synthesized peptides 12- to 18-amino acids long, with overlapping sequences that cover the entire sequence of a protein, covalently linked to a solid support. This unit describes how to construct peptide SPOT arrays, biotinylate recombinant proteins, and conduct overlay assays to identify binding interactions. In addition, directions describing how to analyze results to determine single amino acid binding contributions are included. The two techniques in this unit describe how to scan protein sequences to find binding motifs and how to conduct site-directed mutagenesis studies. Curr. Protoc. Protein Sci. 51:18.10.1-18.10.9. © 2008 by John Wiley & Sons, Inc.
- protein-protein interactions