Unit

UNIT 19.4 Detection of Protein-Protein Interactions by Coprecipitation

  1. Elaine A. Elion

Published Online: 1 AUG 2007

DOI: 10.1002/0471140864.ps1904s49

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Elion, E. A. 2007. Detection of Protein-Protein Interactions by Coprecipitation. Current Protocols in Protein Science. 49:19.4:19.4.1–19.4.10.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 AUG 2007
  2. Published Print: AUG 2007

Abstract

Co-precipitation of proteins from whole cell extracts is a valuable approach to test for physical interactions between proteins of interest. It may be the method of choice, or may be used in conjunction with other procedures to detect protein-protein interactions, such as two-hybrid analyis and co-purification schemes, and tests of physical associations using purified proteins. Curr. Protoc. Protein Sci. 49:19.4.1-19.4.10. © 2007 by John Wiley & Sons, Inc.

Keywords:

  • co-immunoprecipitation;
  • immunoprecipitation;
  • protein interactions;
  • protein tag;
  • antibody;
  • immunoblot