UNIT 19.4 Detection of Protein-Protein Interactions by Coprecipitation
Published Online: 1 AUG 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Elion, E. A. 2007. Detection of Protein-Protein Interactions by Coprecipitation. Current Protocols in Protein Science. 49:19.4:19.4.1–19.4.10.
- Published Online: 1 AUG 2007
- Published Print: AUG 2007
Co-precipitation of proteins from whole cell extracts is a valuable approach to test for physical interactions between proteins of interest. It may be the method of choice, or may be used in conjunction with other procedures to detect protein-protein interactions, such as two-hybrid analyis and co-purification schemes, and tests of physical associations using purified proteins. Curr. Protoc. Protein Sci. 49:19.4.1-19.4.10. © 2007 by John Wiley & Sons, Inc.
- protein interactions;
- protein tag;