Unit

UNIT 19.11 Production and Use of Trimeric Isoleucine Zipper Fusion Proteins to Study Surface Receptor Ligand Interactions

  1. Carsten Watzl

Published Online: 1 MAR 2006

DOI: 10.1002/0471140864.ps1911s43

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Watzl, C. 2006. Production and Use of Trimeric Isoleucine Zipper Fusion Proteins to Study Surface Receptor Ligand Interactions. Current Protocols in Protein Science. 43:19.11:19.11.1–19.11.13.

Author Information

  1. University of Heidelberg, Heidelberg, Germany

Publication History

  1. Published Online: 1 MAR 2006
  2. Published Print: FEB 2006

Abstract

Soluble recombinant forms of surface receptors are commonly used to study the interaction between cell surface receptors and their ligands. By fusing the extracellular domain of the receptor to an isoleucine zipper (ILZ) sequence, it is possible to generate recombinant receptor fusion proteins that form trimers in solution. These ILZ fusion proteins demonstrate several advantages over the commonly used Fc fusion proteins. This unit describes the production and purification of recombinant ILZ fusion proteins. A protocol is also provided for the use of ILZ fusion proteins to study cell surface receptor-ligand interactions on intact cells by flow cytometry.

Keywords:

  • surface receptor;
  • soluble receptor fusion protein;
  • recombinant protein;
  • receptor-ligand interaction;
  • trimer