Unit

UNIT 20.2 Measuring Protein Interactions by Optical Biosensors

  1. Peter Schuck1,
  2. Lisa F. Boyd1,
  3. Peter S. Andersen2

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps2002s17

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Schuck, P., Boyd, L. F. and Andersen, P. S. 2001. Measuring Protein Interactions by Optical Biosensors. Current Protocols in Protein Science. 17:20.2:20.2.1–20.2.22.

Author Information

  1. 1

    National Institutes of Health, Bethesda, Maryland

  2. 2

    University of Maryland, Rockville, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1999

Abstract

This unit gives an introduction to the basic techniques of optical biosensing for measuring equilibrium and kinetics of reversible protein interactions. Emphasis is given to the description of simple and robust approaches that will provide reliable results. How to avoid the most commonly encountered problems and artifacts is also discussed.