UNIT 20.3 Analytical Centrifugation: Equilibrium Approach

  1. Tom Laue

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps2003s18

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Laue, T. 2001. Analytical Centrifugation: Equilibrium Approach. Current Protocols in Protein Science. 18:20.3:20.3.1–20.3.13.

Author Information

  1. University of New Hampshire, Durham, New Hampshire

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1999


The specific interaction of biological molecules with one another is fundamental to the biochemistry of all living things. Equilibrium sedimentation is a classic method of biochemistry that provides first-principle thermodynamic information about the molar mass, association energy, association stoichiometry, and thermodynamic nonideality of molecules in solution. It is one of the most rigorous, powerful, and readily adapted methods for characterizing solution interactions.