Unit

UNIT 20.5 Reduced-Scale Large-Zone Analytical Gel-Filtration Chromatography for Measurement of Protein Association Equilibria

  1. Dorothy Beckett

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.ps2005s18

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Beckett, D. 2001. Reduced-Scale Large-Zone Analytical Gel-Filtration Chromatography for Measurement of Protein Association Equilibria. Current Protocols in Protein Science. 18:20.5:20.5.1–20.5.13.

Author Information

  1. University of Maryland, College Park, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1999

Abstract

The proteasome plays a central role in eukaryotic cells since it is responsible for the degradation of specific proteins involved in a large range of cellular processes. Analysis of proteasome mechanisms of action, or in vitro reconstitution, or dissection of the complex biological pathways in which it partakes, requires a reliable source of pure active proteasome. Although the biologically relevant form of the proteasome is usually considered to be the 26S proteasome, this unit describes different methods for purification and study of both 26S and 20S proteasomes from Saccharomyces cerevisiae cells.