UNIT 20.6 Size-Exclusion Chromatography with On-Line Light Scattering
Published Online: 1 NOV 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Arakawa, T. and Wen, J. 2001. Size-Exclusion Chromatography with On-Line Light Scattering. Current Protocols in Protein Science. 25:20.6:20.6.1–20.6.21.
- Published Online: 1 NOV 2001
- Published Print: SEP 2001
This unit describes the use of size-exclusion chromatography with on-line light scattering, UV absorbance, and refractive index detectors (SEC-LS/UV/RI) to determine: (a) the molecular weight of simple proteins containing no carbohydrates, (b) the molecular weight of glycoproteins, and (c), most importantly, the molecular weight and stoichiometry of protein-protein complexes or protein-carbohydrate complexes. Multiangle light scattering is also discussed.