Unit

UNIT 20.6 Size-Exclusion Chromatography with On-Line Light Scattering

  1. Tsutomu Arakawa1,
  2. Jie Wen2

Published Online: 1 NOV 2001

DOI: 10.1002/0471140864.ps2006s25

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Arakawa, T. and Wen, J. 2001. Size-Exclusion Chromatography with On-Line Light Scattering. Current Protocols in Protein Science. 25:20.6:20.6.1–20.6.21.

Author Information

  1. 1

    Alliance Protein Laboratories, Inc., Thousand Oaks, California

  2. 2

    Amgen Inc., Thousand Oaks, California

Publication History

  1. Published Online: 1 NOV 2001
  2. Published Print: SEP 2001

Abstract

This unit describes the use of size-exclusion chromatography with on-line light scattering, UV absorbance, and refractive index detectors (SEC-LS/UV/RI) to determine: (a) the molecular weight of simple proteins containing no carbohydrates, (b) the molecular weight of glycoproteins, and (c), most importantly, the molecular weight and stoichiometry of protein-protein complexes or protein-carbohydrate complexes. Multiangle light scattering is also discussed.