UNIT 20.10 Circular Dichroism to Study Protein Interactions
Published Online: 1 DEC 2006
Copyright © 2006 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Kelly, S. M. and Price, N. C. 2006. Circular Dichroism to Study Protein Interactions. Current Protocols in Protein Science. 46:20.10:20.10.1–20.10.18.
- Published Online: 1 DEC 2006
- Published Print: NOV 2006
Circular dichroism (CD) is a powerful technique for studying the structures of proteins in solution, as well as structural changes that may occur when proteins bind to ligands. Changes in CD signals accompanying complex formation can be used to determine the strength of the interaction and to provide information about the nature and extent of the conformational changes involved. This unit outlines the type of information available from CD studies and describes how such experiments should be carried out to ensure that reliable information is obtained.
- circular dichroism;
- protein structure;
- conformational change;
- ligand binding