UNIT 20.10 Circular Dichroism to Study Protein Interactions

  1. Sharon M. Kelly,
  2. Nicholas C. Price

Published Online: 1 DEC 2006

DOI: 10.1002/0471140864.ps2010s46

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Kelly, S. M. and Price, N. C. 2006. Circular Dichroism to Study Protein Interactions. Current Protocols in Protein Science. 46:20.10:20.10.1–20.10.18.

Author Information

  1. University of Glasgow, Glasgow, United Kingdom

Publication History

  1. Published Online: 1 DEC 2006
  2. Published Print: NOV 2006


Circular dichroism (CD) is a powerful technique for studying the structures of proteins in solution, as well as structural changes that may occur when proteins bind to ligands. Changes in CD signals accompanying complex formation can be used to determine the strength of the interaction and to provide information about the nature and extent of the conformational changes involved. This unit outlines the type of information available from CD studies and describes how such experiments should be carried out to ensure that reliable information is obtained.


  • circular dichroism;
  • protein structure;
  • conformational change;
  • ligand binding