UNIT 21.2 Papain-like Cysteine Proteases
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Brömme, D. 2001. Papain-like Cysteine Proteases. Current Protocols in Protein Science. 21:21.2:21.2.1–21.2.14.
- Published Online: 1 MAY 2001
- Published Print: SEP 2000
The name “cysteine protease“ refers to the protease's nucleophilic cysteine residue that forms a covalent bond with the carbonyl group of the scissile peptide bond in substrates. The papain-like cysteine proteases, classified as the “C1 family” are the most predominant cysteine proteases. These proteases are found in viruses, plants, primitive parasites, invertebrates, and vertebrates alike. Mammalian papain-like cysteine proteases are also known as cathepsins. This unit discusses cathepsins, and their subcellular and tissue localization, catalytic mechanism, and substrate specificity. Several tables illustrate the properties of the various cathepsins.