UNIT 21.6 Purification of the Eukaryotic 20S Proteasome
Published Online: 1 NOV 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Wilk, S. and Chen, W.-E. 2001. Purification of the Eukaryotic 20S Proteasome. Current Protocols in Protein Science. 25:21.6:21.6.1–21.6.9.
- Published Online: 1 NOV 2001
- Published Print: SEP 2001
The 20S proteasome is the catalytic core of the major extralysosomal proteolytic system of the cell. Combination of the 20S proteasome with a complex of regulatory proteins forms the 26S proteasome, which in turn is responsible for the recognition and degradation of ubiquitin-protein conjugates. As described in this unit, the constitutive form of the 20S proteasome can be conveniently purified as a stable and homogeneous preparation from bovine pituitaries. A support protocol details an enzyme assay used in evaluating proteasomal activity. The 20S proteasome is the catalytic core of the major extralysosomal proteolytic system of the cell.