UNIT 21.7 Serpins (Serine Protease Inhibitors)

  1. Susannah J. Bauman,
  2. Herbert C. Whinna,
  3. Frank C. Church

Published Online: 1 FEB 2002

DOI: 10.1002/0471140864.ps2107s26

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Bauman, S. J., Whinna, H. C. and Church, F. C. 2002. Serpins (Serine Protease Inhibitors). Current Protocols in Protein Science. 26:21.7:21.7.1–21.7.14.

Author Information

  1. The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina

Publication History

  1. Published Online: 1 FEB 2002
  2. Published Print: DEC 2001


Serpins are a class of proteins involved in the regulation of serine and other types of proteases. In humans, the majority of serpins regulate the functions of proteases involved in the body's response to injury. This includes roles in coagulation, fibrinolysis, inflammation, wound healing, and tissue repair. Serpins have been implicated in various animal and human pathologies by the loss of a functional serpin gene through deletion or mutation, which results in a defect in functional protein. Examples of sestorically called antithrombin III) are first described. Then, protocols to determine the second-order rate constant of AT inhibition of thrombin in the absence and presence of heparin are presented. Also provided is a partial list of other serpins and their purification methods.