UNIT 21.7 Serpins (Serine Protease Inhibitors)
Published Online: 1 FEB 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Bauman, S. J., Whinna, H. C. and Church, F. C. 2002. Serpins (Serine Protease Inhibitors). Current Protocols in Protein Science. 26:21.7:21.7.1–21.7.14.
- Published Online: 1 FEB 2002
- Published Print: DEC 2001
Serpins are a class of proteins involved in the regulation of serine and other types of proteases. In humans, the majority of serpins regulate the functions of proteases involved in the body's response to injury. This includes roles in coagulation, fibrinolysis, inflammation, wound healing, and tissue repair. Serpins have been implicated in various animal and human pathologies by the loss of a functional serpin gene through deletion or mutation, which results in a defect in functional protein. Examples of sestorically called antithrombin III) are first described. Then, protocols to determine the second-order rate constant of AT inhibition of thrombin in the absence and presence of heparin are presented. Also provided is a partial list of other serpins and their purification methods.