Unit

UNIT 21.13 Expression, Purification, and Characterization of Caspases

  1. Jean-Bernard Denault,
  2. Guy S. Salvesen

Published Online: 1 FEB 2003

DOI: 10.1002/0471140864.ps2113s30

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Denault, J.-B. and Salvesen, G. S. 2003. Expression, Purification, and Characterization of Caspases. Current Protocols in Protein Science. 30:21.13:21.13.1–21.13.15.

Author Information

  1. The Burnham Institute, La Jolla, California

Publication History

  1. Published Online: 1 FEB 2003
  2. Published Print: DEC 2002

Abstract

This unit describes a protocol to obtain milligram amounts of enzymatically active pure recombinant caspases. Specific details for the expression, purification of caspase-3, -6, -7, -8, -9 and -10 are discussed along with strategies to obtain particular forms (e.g., the zymogen) of some of them.