UNIT 21.13 Expression, Purification, and Characterization of Caspases
Published Online: 1 FEB 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Denault, J.-B. and Salvesen, G. S. 2003. Expression, Purification, and Characterization of Caspases. Current Protocols in Protein Science. 30:21.13:21.13.1–21.13.15.
- Published Online: 1 FEB 2003
- Published Print: DEC 2002
This unit describes a protocol to obtain milligram amounts of enzymatically active pure recombinant caspases. Specific details for the expression, purification of caspase-3, -6, -7, -8, -9 and -10 are discussed along with strategies to obtain particular forms (e.g., the zymogen) of some of them.