Unit

UNIT 21.15 Zymography of Metalloproteinases

  1. Linda Troeberg,
  2. Hideaki Nagase

Published Online: 1 NOV 2004

DOI: 10.1002/0471140864.ps2115s33

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Troeberg, L. and Nagase, H. 2004. Zymography of Metalloproteinases. Current Protocols in Protein Science. 33:21.15:21.15.1–21.15.12.

Author Information

  1. Imperial College London, London, United Kingdom

Publication History

  1. Published Online: 1 NOV 2004
  2. Published Print: AUG 2003

Abstract

Zymography is an electrophoretic technique enabling visualization of the number and approximate size of peptidases in a sample on the basis of their hydrolysis of a protein substrate within the gel. The technique is particularly useful for analyzing the peptidase composition of complex biological samples because visualization depends directly on proteolytic activity. This unit presents a representative zymography protocol for the study of matrix metallopeptidases (MMPs).