UNIT 22.3 Laser Capture Microdissection for Proteome Analysis
Published Online: 1 MAY 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Craven, R. A. and Banks, R. E. 2003. Laser Capture Microdissection for Proteome Analysis. Current Protocols in Protein Science. 31:22.3:22.3.1–22.3.27.
- Published Online: 1 MAY 2003
- Published Print: FEB 2003
Laser capture microdissection is being employed increasingly to isolate specific cell types from tissues, thus overcoming problems of experimental interpretation due to tissue heterogeneity of samples. This unit describes protocols which have been optimized to allow laser capture microdissection of tissues with minimal effect on protein integrity, and their subsequent analysis by techniques including 2D-PAGE, immunoblotting and SELDI, and discusses the relative merits of this approach.