UNIT 22.3 Laser Capture Microdissection for Proteome Analysis

  1. Rachel A. Craven,
  2. Rosamonde E. Banks

Published Online: 1 MAY 2003

DOI: 10.1002/0471140864.ps2203s31

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Craven, R. A. and Banks, R. E. 2003. Laser Capture Microdissection for Proteome Analysis. Current Protocols in Protein Science. 31:22.3:22.3.1–22.3.27.

Author Information

  1. St. James's University Hospital, Leeds, United Kingdom

Publication History

  1. Published Online: 1 MAY 2003
  2. Published Print: FEB 2003


Laser capture microdissection is being employed increasingly to isolate specific cell types from tissues, thus overcoming problems of experimental interpretation due to tissue heterogeneity of samples. This unit describes protocols which have been optimized to allow laser capture microdissection of tissues with minimal effect on protein integrity, and their subsequent analysis by techniques including 2D-PAGE, immunoblotting and SELDI, and discusses the relative merits of this approach.