UNIT 22.4 Preparing Protein Extracts for Quantitative Two-Dimensional Gel Comparison
Published Online: 1 AUG 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Chevallet, M., Tastet, C., Luche, S. and Rabilloud, T. 2004. Preparing Protein Extracts for Quantitative Two-Dimensional Gel Comparison. Current Protocols in Protein Science. 32:22.4:22.4.1–22.4.9.
- Published Online: 1 AUG 2004
- Published Print: MAY 2003
This unit describes basic protocols for efficient and reproducible protein solubilization from a variety of biological samples, including cultured animal cells and tissues, plant cells and tissues, bacteria, nuclei, other subcellular organelles, plasma, serum, and other biological fluids. The optimized extraction process is strongly sample-dependent and cannot be described for every type of sample. Instead, typical protocols are provided as general guidelines and illustrate good starting points for sample-preparation optimization. These solubilization procedures take into account the constraints brought by two-dimensional electrophoresis and are thus well suited for proteomic approaches.
- protein extraction;
- sample preparation;
- two-dimensional electrophoresis;
- protein solubilization