Unit

UNIT 22.4 Preparing Protein Extracts for Quantitative Two-Dimensional Gel Comparison

  1. Mireille Chevallet,
  2. Christophe Tastet,
  3. Sylvie Luche,
  4. Thierry Rabilloud

Published Online: 1 AUG 2004

DOI: 10.1002/0471140864.ps2204s32

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Chevallet, M., Tastet, C., Luche, S. and Rabilloud, T. 2004. Preparing Protein Extracts for Quantitative Two-Dimensional Gel Comparison. Current Protocols in Protein Science. 32:22.4:22.4.1–22.4.9.

Author Information

  1. Départment Résponse et Dynamique, Cellulaire/BioEnergetique Cellulaire et Pathologique, Commissariat à l'Energie Atomique, Grenoble, France

Publication History

  1. Published Online: 1 AUG 2004
  2. Published Print: MAY 2003

Abstract

This unit describes basic protocols for efficient and reproducible protein solubilization from a variety of biological samples, including cultured animal cells and tissues, plant cells and tissues, bacteria, nuclei, other subcellular organelles, plasma, serum, and other biological fluids. The optimized extraction process is strongly sample-dependent and cannot be described for every type of sample. Instead, typical protocols are provided as general guidelines and illustrate good starting points for sample-preparation optimization. These solubilization procedures take into account the constraints brought by two-dimensional electrophoresis and are thus well suited for proteomic approaches.

Keywords:

  • proteomics;
  • protein extraction;
  • sample preparation;
  • two-dimensional electrophoresis;
  • protein solubilization