Unit
UNIT 22.5 Isolation of Organelles and Prefractionation of Protein Extracts Using Free-Flow Electrophoresis
Published Online: 1 AUG 2004
DOI: 10.1002/0471140864.ps2205s32
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
Additional Information
How to Cite
Weber, P. J. A., Weber, G. and Eckerskorn, C. 2004. Isolation of Organelles and Prefractionation of Protein Extracts Using Free-Flow Electrophoresis. Current Protocols in Protein Science. 32:22.5:22.5.1–22.5.21.
Publication History
- Published Online: 1 AUG 2004
- Published Print: MAY 2003
- Abstract
- Article
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Abstract
One of the major obstacles in the analysis of proteomes is the extreme complexity of any particular cell or biological fluid. Free-flow electrophoresis (FFE) is a powerful tool for reduction of this complexity, which is a prerequisite for systematic and comprehensive protein analyses. Protocols are provided in this unit for sample fractionation at two different stages: on the protein level by isoelectric focusing FFE fractionation of crude protein mixtures such as whole cell lysates, and on a subcellular level by zone-electrophoretic FFE purification of organelles.
Keywords:
- Free-flow electrophoresis;
- fractionation;
- organelles;
- protein;
- proteomics;
- isoelectric focusing;
- zone electrophoresis
