Unit
UNIT 23.1 Analysis of Protein Composition Using Multidimensional Chromatography and Mass Spectrometry
Published Online: 1 FEB 2004
DOI: 10.1002/0471140864.ps2301s34
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
Additional Information
How to Cite
Link, A. J., Jennings, J. L. and Washburn, M. P. 2004. Analysis of Protein Composition Using Multidimensional Chromatography and Mass Spectrometry. Current Protocols in Protein Science. 34:23.1:23.1.1–23.1.25.
Publication History
- Published Online: 1 FEB 2004
- Published Print: NOV 2003
This is not the most recent version of the article. View current version (3 NOV 2014)
Abstract
Multidimensional liquid chromatography of peptides produced by protease digestion of complex protein mixtures followed by tandem mass spectrometry can be coupled with automated database searching to identify large numbers of proteins in complex samples. These methods avoid the limitations of gel electrophoresis and in-gel digestions by directly identifying protein mixtures in solution.
