UNIT 23.4 Quantitative Protein Analysis Using Proteolytic [18O]Water Labeling

  1. Kristy J. Reynolds,
  2. Catherine Fenselau

Published Online: 1 FEB 2004

DOI: 10.1002/0471140864.ps2304s34

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Reynolds, K. J. and Fenselau, C. 2004. Quantitative Protein Analysis Using Proteolytic [18O]Water Labeling. Current Protocols in Protein Science. 34:23.4:23.4.1–23.4.8.

Author Information

  1. University of Maryland, College Park, Maryland

Publication History

  1. Published Online: 1 FEB 2004
  2. Published Print: NOV 2003

This is not the most recent version of the article. View current version (1 APR 2014)


This unit describes the procedure for proteolytic [18O]water labeling of peptides in order to quantitate relative protein levels for a comparative proteomic experiment.


  • comparative proteomics;
  • proteolytic 18O labeling;
  • H218O;
  • quantitation