Unit

UNIT 23.4 Quantitative Protein Analysis Using Proteolytic [18O]Water Labeling

  1. Kristy J. Reynolds,
  2. Catherine Fenselau

Published Online: 1 FEB 2004

DOI: 10.1002/0471140864.ps2304s34

Lab Protocol Title

Current Protocols in Protein Science

Current Protocols in Protein Science

Additional Information

How to Cite

Reynolds, K. J. and Fenselau, C. 2004. Quantitative Protein Analysis Using Proteolytic [18O]Water Labeling. Current Protocols in Protein Science. 34:23.4:23.4.1–23.4.8.

Author Information

  1. University of Maryland, College Park, Maryland

Publication History

  1. Published Online: 1 FEB 2004
  2. Published Print: NOV 2003

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Abstract

This unit describes the procedure for proteolytic [18O]water labeling of peptides in order to quantitate relative protein levels for a comparative proteomic experiment.

Keywords:

  • comparative proteomics;
  • proteolytic 18O labeling;
  • H218O;
  • quantitation
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