UNIT 23.5 Protein Microarrays
Published Online: 1 MAR 2005
Copyright © 2005 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Kramer, S., Joos, T. O. and Templin, M. F. 2005. Protein Microarrays. Current Protocols in Protein Science. 39:23.5:23.5.1–23.5.20.
- Published Online: 1 MAR 2005
- Published Print: FEB 2005
This is not the most recent version of the article. View current version (1 AUG 2011)
With the introduction of DNA microarrays as novel analytical tools, the determination of thousands of binding events in one reaction became possible. The developed technology platforms are not limited to nucleic acids, and, in principle, every ligand-binding assay that works on solid phase can be miniaturized and brought into an array format. This unit explains how protein microarrays can be generated using equipment originally designed for DNA microarrays and how multiplexed assays for the quantification of proteins are set up. A protocol that describes a parallelized system for detecting autoantibodies in human serum is included as an example, and it is shown how existing sandwich immunoassays can be miniaturized and performed in array format. The unit also provides some theoretical background and commentary on the problems associated with this still-novel technology.