Unit

UNIT 24.2 Organelle Proteomics

  1. Pierre Lescuyer,
  2. Mireille Chevallet,
  3. Sylvie Luche,
  4. Thierry Rabilloud

Published Online: 1 JUN 2006

DOI: 10.1002/0471140864.ps2402s44

Lab Protocol Title

Current Protocols in Protein Science

Current Protocols in Protein Science

Additional Information

How to Cite

Lescuyer, P., Chevallet, M., Luche, S. and Rabilloud, T. 2006. Organelle Proteomics. Current Protocols in Protein Science. 44:24.2:24.2.1–24.2.9.

Author Information

  1. CEA Grenoble, Grenoble, France

Publication History

  1. Published Online: 1 JUN 2006
  2. Published Print: MAY 2006
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Abstract

This unit describes strategies for studying the proteomes of organelles, which is one example of targeted proteomics. It relies heavily on previously published units dealing with organelle preparation, protein solubilization, and proteomics techniques. A specific commentary for organelle proteomics is provided. Specific protocols for the isolation of nuclei from various sources (cell cultures, tissues) are also provided.

Keywords:

  • proteomics;
  • organelle;
  • nuclei;
  • mitochondria
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