UNIT 26.7 Survey of Protein Engineering Strategies
Published Online: 1 NOV 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Bornscheuer, U. and Kazlauskas, R. J. 2011. Survey of Protein Engineering Strategies. Current Protocols in Protein Science. 66:26.7:26.7.1–26.7.14.
- Published Online: 1 NOV 2011
- Published Print: NOV 2011
Protein engineering is altering the structure of a protein to improve or change its properties. This unit summarizes concepts for protein engineering using rational design, directed evolution, and combinations of them. Different strategies are presented for identifying the best mutagenesis method, how to identify desired variants by screening or selection, and examples for successful applications are given. This should enable researchers to choose the most promising tools to solve their protein engineering challenges. Curr. Protoc. Protein Sci. 66:26.7.1-26.7.14. © 2011 by John Wiley & Sons, Inc.
- protein engineering;
- rational protein design;
- directed evolution;