Unit

UNIT 26.7 Survey of Protein Engineering Strategies

  1. Uwe Bornscheuer1,
  2. Romas J. Kazlauskas2

Published Online: 1 NOV 2011

DOI: 10.1002/0471140864.ps2607s66

Lab Protocol Title

Current Protocols in Protein Science

Current Protocols in Protein Science

Additional Information

How to Cite

Bornscheuer, U. and Kazlauskas, R. J. 2011. Survey of Protein Engineering Strategies. Current Protocols in Protein Science. 66:26.7:26.7.1–26.7.14.

Author Information

  1. 1

    Department of Biotechnology and Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Greifswald, Germany

  2. 2

    Department of Biochemistry, Molecular Biology and Biophysics and the Biotechnology Institute, University of Minnesota, Saint Paul, Minnesota

Publication History

  1. Published Online: 1 NOV 2011
  2. Published Print: NOV 2011
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Abstract

Protein engineering is altering the structure of a protein to improve or change its properties. This unit summarizes concepts for protein engineering using rational design, directed evolution, and combinations of them. Different strategies are presented for identifying the best mutagenesis method, how to identify desired variants by screening or selection, and examples for successful applications are given. This should enable researchers to choose the most promising tools to solve their protein engineering challenges. Curr. Protoc. Protein Sci. 66:26.7.1-26.7.14. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • protein engineering;
  • rational protein design;
  • directed evolution;
  • biocatalysis
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