Unit

UNIT 27.5 Detecting Low-Affinity Extracellular Protein Interactions Using Protein Microarrays

  1. Yi Sun,
  2. Gavin J. Wright

Published Online: 1 APR 2013

DOI: 10.1002/0471140864.ps2705s72

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Sun, Y. and Wright, G. J. 2013. Detecting Low-Affinity Extracellular Protein Interactions Using Protein Microarrays. Current Protocols in Protein Science. 72:27.5:27.5.1–27.5.15.

Author Information

  1. Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom

Publication History

  1. Published Online: 1 APR 2013
  2. Published Print: APR 2013

Abstract

Low-affinity extracellular protein interactions are critical for cellular recognition processes, but are not generally detected by methods that can be applied in a high-throughput manner. This unit describes a protein microarray platform that significantly improves the throughput of assays capable of detecting transient extracellular protein interactions. These methodological improvements now permit screening for novel extracellular receptor-ligand interactions on a genome-wide scale. Curr. Protoc. Protein Sci. 72:27.5.1-27.5.15. © 2013 by John Wiley & Sons, Inc.

Keywords:

  • receptor-ligand pairs;
  • extracellular protein interactions;
  • AVEXIS;
  • adhesion receptors;
  • transient/weak interactions;
  • high-throughput screening;
  • microarray