Unit
UNIT 27.5 Detecting Low-Affinity Extracellular Protein Interactions Using Protein Microarrays
Published Online: 1 APR 2013
DOI: 10.1002/0471140864.ps2705s72
Copyright © 2013 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
Additional Information
How to Cite
Sun, Y. and Wright, G. J. 2013. Detecting Low-Affinity Extracellular Protein Interactions Using Protein Microarrays. Current Protocols in Protein Science. 72:27.5:27.5.1–27.5.15.
Publication History
- Published Online: 1 APR 2013
- Published Print: APR 2013
- Abstract
- Article
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Abstract
Low-affinity extracellular protein interactions are critical for cellular recognition processes, but are not generally detected by methods that can be applied in a high-throughput manner. This unit describes a protein microarray platform that significantly improves the throughput of assays capable of detecting transient extracellular protein interactions. These methodological improvements now permit screening for novel extracellular receptor-ligand interactions on a genome-wide scale. Curr. Protoc. Protein Sci. 72:27.5.1-27.5.15. © 2013 by John Wiley & Sons, Inc.
Keywords:
- receptor-ligand pairs;
- extracellular protein interactions;
- AVEXIS;
- adhesion receptors;
- transient/weak interactions;
- high-throughput screening;
- microarray
