Unit

UNIT 29.13 Applications of Lipid Nanodiscs for the Study of Membrane Proteins by Surface Plasmon Resonance

  1. Meg Trahey1,3,
  2. Mavis Jiarong Li2,3,
  3. Hyewon Kwon2,
  4. Erica L. Woodahl1,
  5. Wynton D. McClary2,
  6. William M. Atkins2

Published Online: 3 AUG 2015

DOI: 10.1002/0471140864.ps2913s81

Lab Protocol Title

Current Protocols in Protein Science

Current Protocols in Protein Science

Additional Information

How to Cite

Trahey, M., Li, M.J., Kwon, H., Woodahl, E.L., McClary, W.D., and Atkins, W.M. 2015. Applications of lipid nanodiscs for the study of membrane proteins by surface plasmon resonance. Curr. Protoc. Protein Sci. 81:29.13.1-29.13.16. doi: 10.1002/0471140864.ps2913s81

Author Information

  1. 1

    Department of Biomedical and Pharmaceutical Sciences, University of Montana, Missoula, Montana

  2. 2

    Department of Medicinal Chemistry, University of Washington, Seattle Washington

  3. 3

    These authors contributed equally to this work

Publication History

  1. Published Online: 3 AUG 2015
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Abstract

Methods for the initial steps of surface plasmon resonance analysis of membrane proteins incorporated in lipid nanodiscs are described. Several types of Biacore sensor chips are available and require distinct strategies to immobilize proteonanodiscs on the chip surface. The procedures for immobilization on three of these chips (NTA, antibody coupled CM5, and L1) are described in this unit and results are demonstrated for a model system with cytochrome P4503A4 (CYP3A4) in nanodiscs binding to a polyclonal anti-CYP3A4 antibody. Advantages and disadvantages of each chip type are considered. © 2015 by John Wiley & Sons, Inc.

Keywords:

  • membrane protein;
  • surface plasmon resonance binding analysis;
  • antibody-receptor binding;
  • lipid nanodisc;
  • biosensor
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