Unit

UNIT 30.4 Ligand-Directed Profiling of Organelles with Internalizing Phage Libraries

  1. Andrey S. Dobroff1,2,8,
  2. Roberto Rangel3,8,
  3. Liliana Guzman-Roja3,8,
  4. Carolina C. Salmeron1,2,
  5. Juri G. Gelovani4,
  6. Richard L. Sidman5,
  7. Cristian G. Bologa6,
  8. Tudor I. Oprea6,
  9. C. Jeffrey Brinker7,
  10. Renata Pasqualini2,9,
  11. Wadih Arap1,9

Published Online: 2 FEB 2015

DOI: 10.1002/0471140864.ps3004s79

Lab Protocol Title

Current Protocols in Protein Science

Current Protocols in Protein Science

Additional Information

How to Cite

Dobroff, A.S., Rangel, R., Guzman-Roja, L., Salmeron, C.C., Gelovani, J.G., Sidman, R.L., Bologa, C.G., Oprea, T.I., Brinker, C.J., Pasqualini, R., and Arap, W. 2015. Ligand-Directed Profiling of Organelles with Internalizing Phage Libraries. Curr. Protoc. Protein Sci. 79:30.4.1-30.4.30. doi: 10.1002/0471140864.ps3004s79

Author Information

  1. 1

    Division of Hematology/Oncology, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico

  2. 2

    Division of Molecular Medicine, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico

  3. 3

    Cancer Research Program, Houston Methodist Research Institute, Houston, Texas

  4. 4

    Department of Biomedical Engineering, Wayne State University, Detroit, Michigan

  5. 5

    Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts

  6. 6

    Translational Informatics Division, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico

  7. 7

    Department of Chemical and Nuclear Engineering, The University of New Mexico Cancer Center, Albuquerque, New Mexico

  8. 8

    These authors contributed equally to this work

  9. 9

    These authors contributed equally as senior authors to this work

Publication History

  1. Published Online: 2 FEB 2015
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Abstract

Phage display is a resourceful tool to, in an unbiased manner, discover and characterize functional protein-protein interactions, create vaccines, and engineer peptides, antibodies, and other proteins as targeted diagnostic and/or therapeutic agents. Recently, our group has developed a new class of internalizing phage (iPhage) for ligand-directed targeting of organelles and to identify molecular pathways within live cells. This unique technology is suitable for applications ranging from fundamental cell biology to drug development. This unit describes the methods for generating and screening the iPhage display system, and explains how to select and validate candidate internalizing homing peptide. © 2015 by John Wiley & Sons, Inc.

Keywords:

  • intracellular targeting;
  • intracellular receptors;
  • mammalian cells;
  • organelles;
  • penetratin;
  • phage display;
  • proteomics;
  • peptides
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