APPENDIX 2E Commonly Used Reagents

Abstract

This collection describes the preparation of buffers and reagents used in the manipulation of proteins.

Buffers and Stock Solutions

• Ammonium acetate, 10 M

• Dithiothreitol (DTT), 1 M

• EDTA (ethylenediamine tetraacetic acid), 0.5 M (pH 8.0)

• Ethidium bromide, 10 mg/ml

• HCl, 1 M

• KCl, 1 M

• MgCl₂, 1 M

• MgSO₄, 1 M

• NaCl, 5 M

• NaOH, 10 M

• Phenol, buffered

• Phosphate-buffered saline (PBS)

• Potassium acetate buffer, 0.1 M

• Potassium phosphate buffer, 0.1 M

• SDS, 20%

• Sodium acetate, 3 M

• Sodium acetate buffer, 0.1 M

• Sodium phosphate buffer, 0.1 M

• SSC (sodium chloride/sodium citrate), 20×

• TAE (Tris/acetate/EDTA) electrophoresis buffer

• TBE (Tris/borate/EDTA) electrophoresis buffer

• TE buffer

• Tris⋅Cl [tris(hydroxymethyl)aminomethane], 1 M