APPENDIX 3C Techniques for Mammalian Cell Tissue Culture

  1. Mary C. Phelan

Published Online: 1 DEC 2006

DOI: 10.1002/0471140864.psa03cs46

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Phelan, M. C. 2006. Techniques for Mammalian Cell Tissue Culture. Current Protocols in Protein Science. 46:3C:A.3C.1–A.3C.18.

Author Information

  1. Molecular Pathology Laboratory Network, Maryville, Tennessee

Publication History

  1. Published Online: 1 DEC 2006
  2. Published Print: NOV 2006


This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.


  • mammalian cells;
  • cell culture;
  • trypsinizing;
  • passaging;
  • hemacytometer