Appendix

APPENDIX 3C Techniques for Mammalian Cell Tissue Culture

  1. Mary C. Phelan

Published Online: 1 DEC 2006

DOI: 10.1002/0471140864.psa03cs46

Lab Protocol Title

Current Protocols in Protein Science

Current Protocols in Protein Science

Additional Information

How to Cite

Phelan, M. C. 2006. Techniques for Mammalian Cell Tissue Culture. Current Protocols in Protein Science. 46:3C:A.3C.1–A.3C.18.

Author Information

  1. Molecular Pathology Laboratory Network, Maryville, Tennessee

Publication History

  1. Published Online: 1 DEC 2006
  2. Published Print: NOV 2006
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Abstract

This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.

Keywords:

  • mammalian cells;
  • cell culture;
  • trypsinizing;
  • passaging;
  • hemacytometer
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