Appendix

APPENDIX 3E Silanizing Glassware

  1. Brian Seed

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.psa03es13

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Seed, B. 2001. Silanizing Glassware. Current Protocols in Protein Science. 13:3E:A.3E.1–A.3E.2.

Author Information

  1. Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1998

Abstract

Untreated glass contains silicate and silanol groups that can act as ion-exchange and nucleophilic centers. To mask these groups and decrease the hydrophilicity of the surface, various reactive silanes are frequently used to coat the glass surface. This appendix describes a procedure for silanizing glassware.