APPENDIX 4B Growth in Liquid or Solid Media
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Protein Science
How to Cite
Elbing, K. L. and Brent, R. 2001. Growth in Liquid or Solid Media. Current Protocols in Protein Science. 13:4B:A.4B.1–A.4B.3.
- Published Online: 1 MAY 2001
- Published Print: SEP 1998
This appendix presents basic procedures for growing overnight (and larger) cultures, monitoring growth, and titering and isolating bacterial cultures by serial dilution. In addition, protocols are provided for isolating single colonies by streaking and spreading a plate. Replica plating and strain storage/retrieval is also detailed.