APPENDIX 4B Growth in Liquid or Solid Media

  1. Karen L. Elbing1,
  2. Roger Brent2

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.psa04bs13

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Elbing, K. L. and Brent, R. 2001. Growth in Liquid or Solid Media. Current Protocols in Protein Science. 13:4B:A.4B.1–A.4B.3.

Author Information

  1. 1

    Clark and Elbing LLP, Boston, Massachusetts

  2. 2

    The Molecular Sciences Institute, Berkeley, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1998


This appendix presents basic procedures for growing overnight (and larger) cultures, monitoring growth, and titering and isolating bacterial cultures by serial dilution. In addition, protocols are provided for isolating single colonies by streaking and spreading a plate. Replica plating and strain storage/retrieval is also detailed.