Appendix

APPENDIX 4C Preparation of Plasmid DNA

  1. JoAnne Engebrecht1,
  2. Roger Brent2

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.psa04cs13

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Engebrecht, J. and Brent, R. 2001. Preparation of Plasmid DNA. Current Protocols in Protein Science. 13:4C:A.4C.1–A.4C.3.

Author Information

  1. 1

    State University of New York, Stony Brook, New York

  2. 2

    The Molecular Scienace Institute, Berkeley, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1998

Abstract

This appendix presents basic procedures for alkaline lysis minipreps, both in tubes and in microtiter plates, and also provides an Alternate Protocol for boiling lysis. A Support Protocol describes storage of plasmid DNA.