Appendix

APPENDIX 4C Preparation of Plasmid DNA

  1. JoAnne Engebrecht1,
  2. Roger Brent2

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.psa04cs13

Lab Protocol Title

Current Protocols in Protein Science

Current Protocols in Protein Science

Additional Information

How to Cite

Engebrecht, J. and Brent, R. 2001. Preparation of Plasmid DNA. Current Protocols in Protein Science. 13:4C:A.4C.1–A.4C.3.

Author Information

  1. 1

    State University of New York, Stony Brook, New York

  2. 2

    The Molecular Scienace Institute, Berkeley, California

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1998
View Full Article (HTML) Get PDF (67K)

Abstract

This appendix presents basic procedures for alkaline lysis minipreps, both in tubes and in microtiter plates, and also provides an Alternate Protocol for boiling lysis. A Support Protocol describes storage of plasmid DNA.

View Full Article (HTML) Get PDF (67K)