Appendix

APPENDIX 4D Introduction of Plasmid DNA into Cells

  1. Christine E. Seidman,
  2. Kevin Struhl

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.psa04ds13

Current Protocols in Protein Science

Current Protocols in Protein Science

How to Cite

Seidman, C. E. and Struhl, K. 2001. Introduction of Plasmid DNA into Cells. Current Protocols in Protein Science. 13:4D:A.4D.1–A.4D.2.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1998

Abstract

This appendix presents a procedure for transformation using calcium chloride, and also an alternate procedure for one-step preparation and transformation of competent cells.