Appendix

APPENDIX 4E Purification and Concentration of DNA from Aqueous Solutions

  1. David Moore

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.psa04es13

Lab Protocol Title

Current Protocols in Protein Science

Current Protocols in Protein Science

Additional Information

How to Cite

Moore, D. 2001. Purification and Concentration of DNA from Aqueous Solutions. Current Protocols in Protein Science. 13:4E:A.4E.1–A.4E.2.

Author Information

  1. Baylor College of Medicine, Houston, Texas

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1998
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Abstract

This unit presents basic procedures for manipulating solutions of single- or double-stranded DNA through purification and concentration steps. Phenol extraction with ethanol precipitation is described along with an alternate procedure of precipitating DNA with isopropanol. A Support Protocol describes concentration of DNA using butanol.

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