Appendix

APPENDIX 4H Hybridization Analysis of DNA Blots

  1. Terry Brown1,
  2. Kevin Struhl (spin-column procedure)2

Published Online: 1 MAY 2001

DOI: 10.1002/0471140864.psa04hs13

Lab Protocol Title

Current Protocols in Protein Science

Current Protocols in Protein Science

Additional Information

How to Cite

Brown, T. and Struhl, K. 2001. Hybridization Analysis of DNA Blots. Current Protocols in Protein Science. 13:4H:A.4H.1–A.4H.9.

Author Information

  1. 1

    University of Manchester Institute of Science and Technology, Manchester, United Kingdom

  2. 2

    Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1998
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Abstract

Restriction endonucleases recognize short DNA sequences and cleave double-stranded DNA at specific sites within or adjacent to the recognition sequences. Restriction endonuclease cleavage of DNA into discrete fragments is one of the most basic procedures in molecular biology. This appendix describes restriction endonucleases and their properties.

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